• br Conflict of interest br

  2018-10-20

  

  Conflict of interest Acknowledgments Introduction In order to understand the molecular and physiological changes in neurons of the brains of individuals with disorders ranging from intellectual disability to autism, it is essential to study live neurons that accurately represent the conditi

• The efficiency of formation of Endocrine Acinar colonies

  2018-10-20

  

  The efficiency of formation of 1° Endocrine/Acinar colonies from unsorted pancreatic apexbio dilution is only ~0.1% (Fig. 2F). CD133highCD71low cells enrich 1° PCFUs-Endocrine/Acinar about 8-fold compared to unsorted cells (Fig. 2F), and the colony-forming efficiency for 1° Endocrine/Acinar colonies

• The pluripotency of MPSII line was confirmed by alkaline

  2018-10-20

  

  The pluripotency of MPSII-4.1 line was confirmed by alkaline phosphatase staining (ALP) and by immunocytochemistry staining (ICC) for endogenous NANOG and -CADHERIN (B) after silencing of the exogenous hOKSM construct. The spontaneous differentiation potential of the iPSC line towards the three ge

• We also found clear differences in the

  2018-10-20

  

  We also found clear differences in the amount of stress fibers between the perivascular derivatives. Abundant stress fibers were found in con-vSMCs located throughout the entire cell body. While hiPSC syn-vSMCs had fewer stress fibers, pericyte derivatives demonstrated the lowest levels of stress fi

• Although there is strong clinical support for

  2018-10-20

  

  Although there is strong clinical support for developing cellular therapies, and the use of such therapies is already reaching clinical translation (Götherström et al., 2014), ethical issues associated with the collection and use of fetal tissue for research and therapy still remain. Concerned polit

• In conclusion we expect that this VP

  2018-10-20

  

  In conclusion, we expect that this VP64dCas9-BFPVP64 platform, which is significantly more potent than previous versions, will encourage the adoption of CRISRP/Cas9-based TF technology to achieve multiplexed gene activation for reprogramming as well as nonreprogramming applications in basic science,

• To achieve a therapeutic effect with genetically corrected

  2018-10-20

  

  To achieve a therapeutic effect with genetically corrected iPSCs for an autologous ex vivo gene therapy approach, we must still overcome several hurdles, such as the successful transplantation of iPSC-derived myogenic cells. Since MYOD1-induced muscle Quercetin dihydrate from iPSCs have the ability

• pkg inhibitor Most AB ECFCs were poorly

  2018-10-20

  

  Most AB-ECFCs were poorly clonogenic, entered rapidly in senescence and had limited functional capacities compared to CB-ECFCs. Moreover, their transcriptional level for the stemness genes DNMT3b, GDF3 and SOX2, that we have previously described (Guillevic et al., 2016), were similar to those found

• In this study we verify

  2018-10-20

  

  In this study, we verify the hypothesis if TRIM28 plays a role in reprogramming of mouse embryonic fibroblasts into iPS cells. We stably silenced the expression of Trim28 in MEFs and submitted them to the reprogramming process by infection with lentiviral vector carrying the expression of Yamanaka f

• A-366 In this study lentiviral vectors were used to ablate

  2018-10-20

  

  In this study, lentiviral vectors were used to ablate CXCR4 and/or CXCR7 expression using shRNAs, and the derived hiPSC-CMs were tested for phenotypic and functional properties due to gene knockdown. Gene expression and flow cytometry studies confirmed the cardiomyocyte phenotype of the differentiat

• Here we report for the first

  2018-10-20

  

  Here, we report for the first time that the mouse eye possesses a primitive c-Kit+ cell that is self-renewing, clonogenic and multipotent, the three critical identifiers of tissue specific stem/progenitor Radicicol (Weissman, 2000). In addition, lineage tracing techniques in vivo demonstrate that th

• Engraftment repopulation of the satellite niche and response

  2018-10-20

  

  Engraftment, repopulation of the satellite niche, and response to additional injury clearly demonstrate that the human CD56+/CD29+ satellite cell population contains muscle stem cells. With respect to engraftment, our experiments resulted in a greater efficiency of human-derived fiber generation (Fi

• br Experimental Procedures br Acknowledgments br Introductio

  2018-10-20

  

  Experimental Procedures Acknowledgments Introduction Hematopoietic stem raas inhibitor (HSCs) are capable of both self-renewal and production of mature blood lineages. Players in this balanced regulation include transcription factors, cell-cycle regulators, signaling molecules, surface rece

• Next we attempted to correct the disease causing mutation in

  2018-10-20

  

  Next we attempted to correct the disease-causing mutation in a fibroblast line isolated from an infant with severe combined immunodeficiency (SCID), caused by mutations in the gene encoding adenosine deaminase (ADA). SCID patients could particularly benefit from a one-step protocol that facilitates

• br Results br Discussion Following transplantation into

  2018-10-20

  

  Results Discussion Following transplantation into immunodeficient mice, immature hESC-derived pancreatic progenitor elastase inhibitor develop over a lengthy maturation period into glucose-responsive, insulin secreting cells (Rezania et al., 2012; Bruin et al., 2013), although this process re

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